Jump to content

Aspergillus oryzae

From Wikipedia, the free encyclopedia
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.

Aspergillus oryzae
A. oryzae growing on rice to make koji
Scientific classification Edit this classification
Domain: Eukaryota
Kingdom: Fungi
Division: Ascomycota
Class: Eurotiomycetes
Order: Eurotiales
Family: Aspergillaceae
Genus: Aspergillus
Species:
A. oryzae
Binomial name
Aspergillus oryzae
(Ahlburg) E. Cohn[1]

Aspergillus oryzae is a mold used in East Asia to saccharify rice, sweet potato, and barley in the making of alcoholic beverages such as sake and shōchū, and also to ferment soybeans for making soy sauce and miso. It is one of the different koji molds ニホンコウジカビ (日本麹黴) (Japanese: nihon kōji kabi) used for food fermentation.

However, in the production of fermented foods of soybeans such as soy sauce and miso, Aspergillus sojae is sometimes used instead of A. oryzae.[2][3] A. oryzae is also used for the production of rice vinegars. Barley kōji (麦麹) or rice kōji (米麹) are made by fermenting the grains with A. oryzae hyphae.[4]

Genomic analysis has led some scholars to believe that the Japanese domesticated the Aspergillus flavus that had mutated and ceased to produce toxic aflatoxins, giving rise to A. oryzae.[5][6][7] While the two fungi share the same cluster of genes that encode for aflatoxin synthesis, this gene cluster is non-functional in A. oryzae.[8] Eiji Ichishima of Tohoku University called the kōji fungus a "national fungus" (kokkin) in the journal of the Brewing Society of Japan, because of its importance not only for making the kōji for sake brewing, but also for making the kōji for miso, soy sauce, and a range of other traditional Japanese foods. His proposal was approved at the society's annual meeting in 2006.[9]

The Japanese word kōji (麹) is used in several meanings, and in some cases it specifically refers to A. oryzae and A. sojae,[2][10] while in other cases it refers to all molds used in fermented foods, including Monascus purpureus and other molds, so care should be taken to avoid confusion.[11]

Properties desirable in sake brewing and testing

The following properties of A. oryzae strains are important in rice saccharification for sake brewing:[12]

Two of the key enzyme groups secreted by A. oryzae are pectinase and peptidase. Pectinase drives starch hydrolysis by breaking down the pectin in the cell walls of plant materials like soybeans, in the case of miso and soy sauce production, while peptidases like leucine aminopeptidase cleave amino acids from proteins and polypeptides like glutamic acid, an amino acid that contributes to the characteristic umami flavor of these fermented soybean products.[13]

A. oryzae secretes a number of salt-tolerant alkaline proteases which makes it particularly stable in the high-sodium conditions required for the production of miso and soy sauce. The strain A. oryzae RIB40, for example, appears to have specific salt tolerance genes that regulate K+ transport.[14][15]

Varieties used for shōchū making

Three varieties of kōji mold are used for making shōchū, each with distinct characteristics.[16][17][18]

Genichirō Kawachi (1883 -1948), who is said to be the father of modern shōchū and Tamaki Inui (1873 -1946), a lecturer at University of Tokyo succeeded in the first isolation and culturing of aspergillus species such as A. kawachii, A. awamori, and a variety of subtaxa of A. oryzae, which led to great progress in producing shōchū in Japan. Since then, aspergillus developed by Kawachi has also been used for soju and makgeolli in Korea.[19][20]

  • Yellow kōji (A. oryzae) is used to produce sake, and at one time all honkaku shōchū. However, yellow kōji is extremely sensitive to temperature; its moromi can easily sour during fermentation. This makes it difficult to use in warmer regions such as Kyūshū, and gradually black and white kōji became more common in production of shōchū. Its strength is that it gives rise to a rich, fruity, refreshing taste, so despite the difficulties and great skill required, it is still used by some manufacturers. It is popular amongst young people who previously had no interest in typically strong potato shōchū, playing a role in its recent revival. Thus, white and black kōji are mainly used in the production of shōchū, but only yellow kōji (A. oryzae) is usually used in the production of sake.
  • White kōji (A. kawachii) was discovered as a mutation from black kōji by Genichirō Kawachi in 1918.[21] This effect was researched and white kōji was successfully grown independently. White kōji is easy to cultivate and its enzymes promote rapid saccharization; as a result, it is used to produce most shōchū today. It gives rise to a drink with a refreshing, mild, sweet taste.
  • Black kōji (A. luchuensis) is mainly used to produce shōchū and awamori. In 1901, Tamaki Inui, lecturer at University of Tokyo succeeded in the first isolating and culturing.[22][23] In 1910, Genichirō Kawachi succeeded for the first time in culturing var. kawachi, a variety of subtaxa of A. awamori. This improved the efficiency of shōchū production.[19] It produces plenty of citric acid which helps to prevent the souring of the moromi. Of all three kōji, it most effectively extracts the taste and character of the base ingredients, giving its shōchū a rich aroma with a slightly sweet, mellow taste. Its spores disperse easily, covering production facilities and workers' clothes in a layer of black. Such issues led to it falling out of favour, but due to the development of new kuro-kōji (NK-kōji) in the mid-1980s,[24] interest in black kōji resurged amongst honkaku shōchū makers because of the depth and quality of the taste it produced. Several popular brands now explicitly state they use black kōji on their labels.

Genome

Initially kept secret, the A. oryzae genome was released by a consortium of Japanese biotechnology companies[25] in late 2005.[26] The eight chromosomes together comprise 37 million base pairs and 12 thousand predicted genes. The genome of A. oryzae is thus one-third larger than that of two related Aspergillus species, the genetics model organism A. nidulans and the potentially dangerous A. fumigatus.[27] Many of the extra genes present in A. oryzae are predicted to be involved in secondary metabolism. The sequenced strain isolated in 1950 is called RIB40 or ATCC 42149; its morphology, growth, and enzyme production are typical of strains used for sake brewing.[28]

The increased number of genes in Aspergillus oryzae are responsible for the function of proteins and cellular processes such as hydrolase, transporters, and metabolism. The extensive array of secretory hydrolase and transporters allows the mold to break down or secrete various compounds effectively. Typically, when A. oryzae exposed to high concentrations of foods like rice, soybean, wheat, etc. during fermentation, its growth may be negatively affected. However, over time this may potentially allow the kōji to gain new transporters due to the environment's conditions.[29]

Although A. oryzae is closely related A. flavus and A. parasiticus, which are known to secrete toxins called aflatoxins that cause severe food poisoning,[30] the kōji mold has not been found to produce those toxins.[30][31] Furthermore, no carcinogenic substances have been discovered in the mold.[31] A study has shown that even when A. oryzae is put under conditions favorable to express and secrete aflatoxin, the aflatoxin genes in A. oryzae were not expressed.[32]

Use in biotechnology

Trans-resveratrol can be efficiently cleaved from its glucoside piceid through the process of fermentation by A. oryzae.[33] "Flavourzyme", a protease blend derived from A. oryzae, is used to produce enzyme-hydrolyzed vegetable protein.[34]

A. oryzae is hard to study due to difficulties in conventional genetic manipulation. This is because A. oryzae have cell walls that are difficult to break down which makes gene insertion/editing complicated. However, scientists have recently started utilizing CRISPR/Cas9 in A. oryzae. This increased mutation rates in the genome which was not possible in the past since the mold only reproduced asexually.[35]

Secondary metabolites

A. oryzae is a good choice as a secondary metabolite factory because of its relatively few endogenous secondary metabolites. Transformed types can produce: polyketide synthase-derived 1,3,6,8-tetrahydroxynaphthalene, alternapyrone, and 3-methylorcinaldehyde; citrinin; terrequinone A; tennelin, pyripyropene, aphidicolin, terretonin, and andrastin A by plasmid insertion; paxilline and aflatrem by co-transformation; and aspyridone, originally from A. nidulans, by Gateway cloning.[36][37]

History of 麹 in a broad sense

麹 (Chinese qū, Japanese kōji) which means mold used in fermented foods, was first mentioned in the Zhouli (Rites of the Zhou dynasty) in China in 300 BCE. Its development is a milestone in Chinese food technology, for it provides the conceptual framework for three major fermented soy foods: soy sauce, jiang/miso, and douchi, not to mention grain-based wines (including Japanese sake and Chinese huangjiu) and li (the Chinese forerunner of Japanese amazake).[38][28]

  • Conidiophores with conidia of the microscopic fungus A. oryzae under light microscope
    Conidiophores with conidia of the microscopic fungus A. oryzae under light microscope
  • Four Aspergillus colonies grown at 37 °C (99 °F) for three days on rich media. The bottom two are A. oryzae strains.
    Four Aspergillus colonies grown at 37 °C (99 °F) for three days on rich media. The bottom two are A. oryzae strains.

See also

References

  1. ^ Index Fungorum
  2. ^ a b Kenichiro Matsushima. しょうゆづくりの歩みと麹菌の関わり (PDF) (in Japanese). The Society for Biotechnology, Japan. p. 75. Archived from the original (PDF) on 26 July 2020.
  3. ^ 麹菌ゲノム解読 Kikkoman Corporation
  4. ^ Parmjit S. Panesar, Biotechnology in Agriculture and Food Processing: Opportunities and Challenges CRC Press (2014)
  5. ^ Katsuhiko Kitamoto. 麹菌物語 (PDF) (in Japanese). The Society for Biotechnology, Japan. p. 424. Archived from the original (PDF) on 31 October 2022.
  6. ^ Katsuhiko Kitamoto. 家畜化された微生物、麹菌 その分子細胞生物学的解析から見えてきたこと (PDF) (in Japanese). The Society of Yeast Scientists. p. 2. Archived from the original (PDF) on 13 November 2022.
  7. ^ Kiyoko Hayashi (19 July 2021). 日本の発酵技術と歴史 (in Japanese). Discover Japan Inc. Archived from the original on 10 November 2022.
  8. ^ Kiyota, Takuro; Hamada, Ryoko; Sakamoto, Kazutoshi; Iwashita, Kazuhiro; Yamada, Osamu; Mikami, Shigeaki (May 2011). "Aflatoxin non-productivity of Aspergillus oryzae caused by loss of function in the aflJ gene product". Journal of Bioscience and Bioengineering. 111 (5): 512–517. doi:10.1016/j.jbiosc.2010.12.022. ISSN 1389-1723. PMID 21342785.
  9. ^ Fujita, Chieko, Tokyo Foundation Koji, an Aspergillus Archived 2009-05-22 at the Wayback Machine
  10. ^ Kenichiro Matsushima. 醤油づくりと麹菌の利用ー今までとこれからー (in Japanese). p. 643. Archived from the original on 21 June 2022.
  11. ^ 麹のこと Marukome co., ltd.
  12. ^ Kitamoto, Katsuhiko (2002). Molecular Biology of the Koji Molds. Advances in Applied Microbiology. Vol. 51. pp. 129–153. doi:10.1016/S0065-2164(02)51004-2. ISBN 9780120026531. PMID 12236056. Retrieved 2008-01-03.[dead link‍]
  13. ^ Lin, Weimin; Song, Jiajia; Hu, Wenfeng; Miao, Jianyin; Gao, Xiangyang (2016). "Relationship Between Extracellular Cellulase, Pectinase and Xylanase Activity of Isolated Aspergillus oryzae Strains Grown on Koji and the Umami-Tasting Amino Acid Content of Soy Sauce". Food Biotechnology. 30 (4): 278–291. doi:10.1080/08905436.2016.1244768. ISSN 0890-5436. S2CID 88669475.
  14. ^ Wang, D.; Zheng, Z. Y.; Feng, J.; Zhan, X. B.; Zhang, L. M.; Wu, J. R.; Lin, C. C. (2013-07-04). "A high salt tolerant neutral protease from Aspergillus Oryzae: Purification, characterization and kinetic properties". Applied Biochemistry and Microbiology. 49 (4): 378–385. doi:10.1134/S0003683813040170. ISSN 1608-3024. S2CID 254189777.
  15. ^ Zhao, Guozhong; Yao, Yunping; Wang, Chunling; Hou, Lihua; Cao, Xiaohong (2013-06-17). "Comparative genomic analysis of Aspergillus oryzae strains 3.042 and RIB40 for soy sauce fermentation". International Journal of Food Microbiology. 164 (2): 148–154. doi:10.1016/j.ijfoodmicro.2013.03.027. ISSN 0168-1605. PMID 23673060.
  16. ^ "In-depth". Retrieved 2007-01-24. (Japanese)
  17. ^ "What is Shochu?". Archived from the original on 2007-09-28. Retrieved 2007-01-24.
  18. ^ "Other terminology relating to Shochu and Awamori". Retrieved 2007-01-27. (Japanese)
  19. ^ a b "初代 河内源一郎（1883～1948）". Kawauchi-kin honpo. Archived from the original on 7 May 2020. Retrieved 9 April 2023.
  20. ^ "元祖 源一郎さんの生マッコリ". Kawauchi-kin honpo. Archived from the original on 7 May 2020. Retrieved 9 April 2023.
  21. ^ Taiki Futagami (May 2022). "The white koji fungus Aspergillus luchuensis mut. kawachii". Bioscience, Biotechnology, and Biochemistry. 86 (5). Japan Society for Bioscience, Biotechnology, and Agrochemistry: 574–584. doi:10.1093/bbb/zbac033. PMID 35238900.
  22. ^ Osamu Yamada; Masayuki Machida; Akira Hosoyama; Masatoshi Goto; Toru Takahashi; Taiki Futagami; Youhei Yamagata; et al. (December 2016). "Genome sequence of Aspergillus luchuensis NBRC 4314". DNA Research. 23 (6). Oxford University Press: 507–515. doi:10.1093/dnares/dsw032. PMC 5144674. PMID 27651094.
  23. ^ Takeo Koizumi (5 April 2018). "黒麹菌の役割 発酵中の雑菌繁殖防ぐ". Okinawa Times. Archived from the original on 4 April 2023. Retrieved 10 April 2023.
  24. ^ "Shochu Circle". Archived from the original on 2007-12-10. Retrieved 2007-12-11.
  25. ^ Goffeau, André (December 2005). "Multiple moulds". Nature. 438 (7071): 1092–1093. doi:10.1038/4381092b. PMID 16371993.
  26. ^ Machida, Masayuki; et al. (December 2005). "Genome sequencing and analysis of Aspergillus oryzae". Nature. 438 (7071): 1157–1161. Bibcode:2005Natur.438.1157M. doi:10.1038/nature04300. PMID 16372010.
  27. ^ Galagan JE, et al. (December 2005). "Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae". Nature. 438 (7071): 1105–1115. Bibcode:2005Natur.438.1105G. doi:10.1038/nature04341. PMID 16372000.
  28. ^ a b Rokas, A. (2009). "The effect of domestication on the fungal proteome". Trends in Genetics. 25 (2): 60–63. doi:10.1016/j.tig.2008.11.003. PMID 19081651.
  29. ^ Machida, M.; Yamada, O.; Gomi, K. (August 2008). "Genomics of Aspergillus oryzae: Learning from the History of Koji Mold and Exploration of Its Future". DNA Research. 15 (4): 173–183. doi:10.1093/dnares/dsn020. ISSN 1340-2838. PMC 2575883. PMID 18820080.
  30. ^ a b Murakami, Hideya (1971). "Classification of the Koji Mold". The Journal of General and Applied Microbiology. 17 (4): 281–309. doi:10.2323/jgam.17.281.
  31. ^ a b Barbesgaard, Peder; Heldt-Hansen, HansPeter; Diderichsen, Børge (February 1992). "On the safety of Aspergillus oryzae: a review". Applied Microbiology and Biotechnology. 36 (5): 569–572. doi:10.1007/BF00183230. ISSN 0175-7598. PMID 1368061. S2CID 7348198.
  32. ^ Wilkinson, Heather; Tsitsigiannis, Dimitrios; Zhang, Yongqiang; Keller, Nancy (2004-08-30). Secondary Metabolite Gene Clusters. CRC Press. pp. 355–385. ISBN 978-0-8247-5655-0. {{cite book}}: |work= ignored (help)
  33. ^ Wang, H.; Liu, L.; Guo, Y. -X.; Dong, Y. -S.; Zhang, D. -J.; Xiu, Z. -L. (2007). "Biotransformation of piceid in Polygonum cuspidatum to resveratrol by Aspergillus oryzae". Applied Microbiology and Biotechnology. 75 (4): 763–768. doi:10.1007/s00253-007-0874-3. PMID 17333175. S2CID 13139293.
  34. ^ Merz, Michael; Eisele, Thomas; Berends, Pieter; Appel, Daniel; Rabe, Swen; Blank, Imre; Stressler, Timo; Fischer, Lutz (17 June 2015). "Flavourzyme, an Enzyme Preparation with Industrial Relevance: Automated Nine-Step Purification and Partial Characterization of Eight Enzymes". Journal of Agricultural and Food Chemistry. 63 (23): 5682–5693. doi:10.1021/acs.jafc.5b01665. PMID 25996918.
  35. ^ Jin, Feng-Jie; Hu, Shuang; Wang, Bao-Teng; Jin, Long (2021-02-23). "Advances in Genetic Engineering Technology and Its Application in the Industrial Fungus Aspergillus oryzae". Frontiers in Microbiology. 12: 644404. doi:10.3389/fmicb.2021.644404. ISSN 1664-302X. PMC 7940364. PMID 33708187.
  36. ^ Anyaogu, Diana Chinyere; Mortensen, Uffe Hasbro (2015-02-10). "Heterologous production of fungal secondary metabolites in Aspergilli". Frontiers in Microbiology. 6. Frontiers: 77. doi:10.3389/fmicb.2015.00077. ISSN 1664-302X. PMC 4322707. PMID 25713568.
  37. ^ Atanasov, Atanas G.; Zotchev, Sergey B.; Dirsch, Verena M.; Supuran, Claudiu T.; et al. (The International Natural Product Sciences Taskforce) (2021-01-28). "Natural products in drug discovery: advances and opportunities". Nature Reviews Drug Discovery. 20 (3). Nature Portfolio: 200–216. doi:10.1038/s41573-020-00114-z. ISSN 1474-1776. PMC 7841765. PMID 33510482.
  38. ^ Shurtleff, W.; Aoyagi, A. History of Koji - Grains and/or Soybeans Enrobed with a Mold Culture (300 BCE to 2012). Lafayette, California: Soyinfo Center. 660 pp. (1,560 references; 142 photos and illustrations, Free online)
Retrieved from "https://en.wikipedia.org/enwiki/w/index.php?title=Aspergillus_oryzae&oldid=1258085888"