File:Label-free Localisation Microscopy SPDM - Super Resolution Microscopy Christoph Cremer.jpg
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Summary
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English: First description of 50 nm optical resolution of cellular structures in label-free cells:
Interestingly SPDM revealed cellular objects not detectable under conventional light microscopy Christoph Cremer, emeritus at Heidelberg university [1] Comprehensive studies published in Micron, 2010: The paper describes for the first time that optical resolution of cellular structures in the lamda/10 range (about 50 nm) can be achieved even in label-free cells using the localization microscopy SPDM (Spectral Precision Distance/Position Determination Microscopy). Beside a substantial resolution improvement of autofluorescent structures, SPDM revealed cellular objects with are not detectable under conventional fluorescence imaging conditions. Rainer Kaufmann, Patrick Müller, Michael Hausmann, Christoph Cremer: Imaging label-free intracellular structures by localisation microscopy. Micron (2010), doi:10.1016/j.micron.2010.03.06 |
| Date | |
| Source | Own work |
| Author | Andy Nestl |
Gallery
- Super Resolution Microscopy - Localisation Microscopy
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Breast Cancer Cells: 3D Dual Color Super Resolution Microscopy of Her2 and Her3 & cluster calculations -
Single YFP molecule detection in a human cancer cell. Typical distance measurements 15 nm -
Co- localisation microscopy with GFP and RFP fusion proteins (nucleus of a bone cancer cell) 120.000 localized molecules in a widefield area(470 µm2) -
Label-free Localisation Microscopy SPDM - Super Resolution Microscopy reveals prior undetebable intracellular structures -
Investigation of human eye tissue, affected by macular degeneration AMD -
Virus Super Resolution Microscopy SPDM Cremer/Wege labs
Licensing
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Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled GNU Free Documentation License. |
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- ↑ https://www.physik.uni-heidelberg.de/personen/lsf.php?details=1537 |titel=Fakultät für Physik und Astronomie |abruf=2020-10-01
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 13:20, 1 December 2011 | | 2,012 × 1,280 (1.55 MB) | Andy Nestl |
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