Examine individual changes

'[[Image:Tissue culture vials nci-vol-2142-300.jpg|thumb|Vials containing tissue culture [[growth medium]] which provides nourishment to growing cells.]] '''Tissue culture''' is the growth of [[biological tissue|tissues]] and/or [[cell (biology)|cells]] separate from the organism. This is typically facilitated via use of a liquid, semi-solid, or solid [[growth medium|growth media]], such as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, while the more specific term [[plant tissue culture]] is used for plants. ==''''Italic text'''Bold text''' tissue culture is a process whereby a small piece of living tissue from isolate a cell to the organism to cultivate the in vitro condition == Introduction == In 1885 [[Wilhelm Roux]] removed a portion of the [[medulla]]ry plate of an [[embryo]]nic [[chicken]] and maintained it in a warm [[saline solution]] for several days, establishing the basic principle of tissue culture. In 1907 the zoologist [[Ross Granville Harrison]] demonstrated the growth of frog nerve cell processes in a medium of clotted lymph. ==Modern usage== {{main|cell culture}} In modern usage, "tissue culture" generally refers to the growth of [[eukaryote|eukaryotic]] cells ''in vitro''. It is often used interchangeably with '''''cell culture''''' to specifically describe the ''in vitro'' culturing of sperm donor cells. However, "tissue culture" can also be used to refer to the culturing of tissue pieces, i.e. [[explant culture]] or whole organs, i.e. [[organ culture]]. It is a tool for the study of animal cell biology in vitro model of cell growth to allow a highly selective environment which is easily manipulated (used to optimize cell signaling pathways). ==See also== * [[Cell culture]] * [[Organ culture]] * [[Electric cell-substrate impedance sensing]] ==External links== *[http://www.research.umbc.edu/~jwolf/method5.htm www.research.umbc.edu/~jwolf/method5.htm] - how-to guide for biology students *[http://www.accessexcellence.org/LC/ST/st2bgplant.html Plant Tissue Culture] - way to "xerox" a plant *[http://www.celos.sr.org/dept/Tissue%20Culture/index.asp CELOS] - a division of CELOS (Center for Agricultural Research in Suriname) [[Category:Histology]] [[Category:Laboratory techniques]] [[Category:Cell cultures]] {{Cell-biology-stub}} [[de:Gewebekultur]] [[fr:Culture in vitro]] [[id:Kultur jaringan]] [[pt:Cultura de tecidos]] [[zh:组织培養]]'