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Original article:
HaeIII is one of many restriction enzymes (endonucleases). It is a restriction enzyme used in molecular biology laboratories. It was the third endonuclease to be isolated from the Haemophilus aegyptius bacteria. The enzyme's recognition site—the place where it cuts DNA molecules—is the GGCC nucleotide sequence which means it cleaves DNA at the site 5′-GG/CC-3. This enzyme's gene has been sequenced and cloned. This is done to make DNA fragments in blunt ends. HaeIII is not effective for single stranded DNA cleavage.
Haemophilus aegyptius also carries a methylase dubbed HaeIIIM (P20589) that methylates the internal cytosines in the GGCC sequence. It protects sequences from being cut by HaeIII, and forms a restriction modification system.
- ^ HaeIII Restriction Enzyme
- Rawn, J. David (1989). Biochemistry, International Edition. ISBN 0-89278-405-9
- NCBI Sequence Viewer.
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Revised:
(Hae lll is from haemophilus aegyptius Hae lll is a restriction enzyme used in molecular biology. It cleaves DNA at the site 5′-GG/CC-3. This is done to make DNA fragments in blunt ends. Hae lll is not effective for single stranded DNA cleavage) --> edited below
HaeIII is one of many restriction enzymes (endonucleases). It is a restriction enzyme used in molecular biology laboratories. It was the third endonuclease to be isolated from the Haemophilus aegyptius bacteria. The enzyme's recognition site—the place where it cuts DNA molecules—is the GGCC nucleotide sequence which means it cleaves DNA at the site 5′-GG/CC-3. This enzyme's gene has been sequenced and cloned. This is done to make DNA fragments in blunt ends. HaeIII is not effective for single stranded DNA cleavage.
<<Properties>>
HaeIII has a molecular weight of 37126. After a 2-10-fold of Hae III takes place, there is overdigestion of a DNA substrate. This results in 100% being cut, more than 50% of fragments being ligated, and more than 95% being recut. Heat inactivation comes about at 80 °C for 20 minutes.
<<history>>
HaeIII along with other restriction enzymes were discovered in 1970 by Werner Arber and Matthew Meselson.
<<Function>>
The enzyme cleaves the DNA at the positions where the GGCC sequence is found. The cleavage occurs between the second and the third nucleotides (G and C). The resulting DNA fragments are known as restriction fragments. HaeIII cuts both strands of DNA in the same location, yielding restriction fragments with blunt ends. Heat denaturation occurs at 80°C after 20 minutes.
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