PANC-1:修订间差异
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'''PANC-1'''是[[人類]][[胰腺癌]] |
'''PANC-1'''是[[人類]][[胰腺癌]][[細胞系]]<ref name=a>{{cite journal |last1=Lieber |first1=M |last2=Mazzetta |first2=J |last3=Nelson-Rees |first3=W |last4=Kaplan |first4=M |last5=Todaro |first5=G |title=Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. |journal=International journal of cancer |date=1975-05-15 |volume=15 |issue=5 |pages=741-7 |doi=10.1002/ijc.2910150505 |pmid=1140870 |url=https://pubmed.ncbi.nlm.nih.gov/1140870-establishment-of-a-continuous-tumor-cell-line-panc-1-from-a-human-carcinoma-of-the-exocrine-pancreas/ |accessdate=2020-01-12 |archive-date=2020-01-12 |archive-url=https://web.archive.org/web/20200112052812/https://pubmed.ncbi.nlm.nih.gov/1140870-establishment-of-a-continuous-tumor-cell-line-panc-1-from-a-human-carcinoma-of-the-exocrine-pancreas/ }}</ref>,最初是分離一名56歲[[男性]]胰腺癌[[患者]]。PANC-1細胞可以[[遠端轉移]],但[[細胞分化]]的能力差<ref name=b>{{cite journal |last1=Deer |first1=EL |last2=González-Hernández |first2=J |last3=Coursen |first3=JD |last4=Shea |first4=JE |last5=Ngatia |first5=J |last6=Scaife |first6=CL |last7=Firpo |first7=MA |last8=Mulvihill |first8=SJ |title=Phenotype and genotype of pancreatic cancer cell lines. |journal=Pancreas |date=2010-05 |volume=39 |issue=4 |pages=425-35 |doi=10.1097/MPA.0b013e3181c15963 |pmid=20418756 |url=https://pubmed.ncbi.nlm.nih.gov/20418756-phenotype-and-genotype-of-pancreatic-cancer-cell-lines/ |accessdate=2020-01-12 |author= |archive-url=https://web.archive.org/web/20200112052812/https://pubmed.ncbi.nlm.nih.gov/20418756-phenotype-and-genotype-of-pancreatic-cancer-cell-lines/ |archive-date=2020-01-12 |dead-url=yes }}</ref>,需要52個[[小時]]才能增加一倍的種群。PANC-1細胞的模態[[染色體]]數為63,包括3個獨特的{{le|標記染色體|Marker chromosome}}和1個小環狀染色體,並且顯示出慢遷移性類型的G6PD<ref name=a/>。目前已知PANC-1細胞具有上皮形態及會粘附在細胞培養瓶中。 只要適當地提高細胞的[[溫度]],細胞就會在培養物中再度生長。PANC-1細胞有結塊的趨勢,而進行[[胰蛋白酶]]的處理則可以避免這種現象<ref>{{cite web |author1=Thompson Evonne |title=Cell Growth Protocol for PANC-1 cell line |url=https://genome.ucsc.edu/encode/protocols/cell/human/PANC-1_Myers_protocol.pdf |publisher=Myers Laboratory |accessdate=2020-01-12 |archiveurl=https://web.archive.org/web/20170918085337/http://genome.ucsc.edu/encode/protocols/cell/human/PANC-1_Myers_protocol.pdf |archivedate=2017-09-18 |language=en |date=2009-07-22 |dead-url=no }}</ref>。PANC-1細胞已被用於[[研究]][[角蛋白]]重組在癌細胞遷移過程中的作用,以及[[鈣]]介導的[[肌動蛋白]]在響應生理變化時的重置<ref name=b/>。 |
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== 參考資料 == |
== 參考資料 == |
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== 外部連結 == |
== 外部連結 == |
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*[https:// |
*[https://www.cellosaurus.org/CVCL_0480 Cellosaurus entry for PANC-1]{{Wayback|url=https://web.expasy.org/cellosaurus/CVCL_0480 |date=20200112052809 }} |
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[[Category:人類細胞系]] |
[[Category:人類細胞系]] |
2022年9月11日 (日) 14:20的最新版本
PANC-1是人類胰腺癌細胞系[1],最初是分離一名56歲男性胰腺癌患者。PANC-1細胞可以遠端轉移,但細胞分化的能力差[2],需要52個小時才能增加一倍的種群。PANC-1細胞的模態染色體數為63,包括3個獨特的標記染色體和1個小環狀染色體,並且顯示出慢遷移性類型的G6PD[1]。目前已知PANC-1細胞具有上皮形態及會粘附在細胞培養瓶中。 只要適當地提高細胞的溫度,細胞就會在培養物中再度生長。PANC-1細胞有結塊的趨勢,而進行胰蛋白酶的處理則可以避免這種現象[3]。PANC-1細胞已被用於研究角蛋白重組在癌細胞遷移過程中的作用,以及鈣介導的肌動蛋白在響應生理變化時的重置[2]。
參考資料
[编辑]- ^ 1.0 1.1 Lieber, M; Mazzetta, J; Nelson-Rees, W; Kaplan, M; Todaro, G. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas.. International journal of cancer. 1975-05-15, 15 (5): 741–7 [2020-01-12]. PMID 1140870. doi:10.1002/ijc.2910150505. (原始内容存档于2020-01-12).
- ^ 2.0 2.1 Deer, EL; González-Hernández, J; Coursen, JD; Shea, JE; Ngatia, J; Scaife, CL; Firpo, MA; Mulvihill, SJ. Phenotype and genotype of pancreatic cancer cell lines.. Pancreas. 2010-05, 39 (4): 425–35 [2020-01-12]. PMID 20418756. doi:10.1097/MPA.0b013e3181c15963. (原始内容存档于2020-01-12).
- ^ Thompson Evonne. Cell Growth Protocol for PANC-1 cell line (PDF). Myers Laboratory. 2009-07-22 [2020-01-12]. (原始内容存档 (PDF)于2017-09-18) (英语).