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异硫氰酸荧光素：修订间差异

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第10行： 第10行：
| ImageCaptionR1 = 6-FITC
| ImageCaptionR1 = 6-FITC
| IUPACName =
| IUPACName =
| OtherNames = FITC<br>5-Isomer: FITC-I<br>6-Isomer: FITC-II
| OtherNames = FITC<br>5-异构体：FITC-I<br>6-异构体：FITC-II
| SystematicName =
| SystematicName =
| Section1 = {{Chembox Identifiers
| Section1 = {{Chembox Identifiers
| ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}}
| ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}}
| ChemSpiderID = 17686
| ChemSpiderID = 17686
| ChemSpiderID_Comment= (5-isomer)
| ChemSpiderID_Comment= 5-异构体
| ChemSpiderID1_Ref = {{chemspidercite|correct|chemspider}}
| ChemSpiderID1_Ref = {{chemspidercite|correct|chemspider}}
| ChemSpiderID1 = 2043359
| ChemSpiderID1 = 2043359
| ChemSpiderID1_Comment= (6-isomer)
| ChemSpiderID1_Comment=6-异构体
| StdInChI_Ref = {{stdinchicite|correct|chemspider}}
| StdInChI_Ref = {{stdinchicite|correct|chemspider}}
| InChI = 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
| InChI = 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
第29行： 第29行：
| CASNo_Ref = {{cascite|correct|CAS}}
| CASNo_Ref = {{cascite|correct|CAS}}
| CASNo = 27072-45-3
| CASNo = 27072-45-3
| CASNo_Comment = (mixture)
| CASNo_Comment =混合物
| CASNo1_Ref = {{cascite|correct|CAS}}
| CASNo1_Ref = {{cascite|correct|CAS}}
| CASNo1 = 3326-32-7
| CASNo1 = 3326-32-7
| CASNo1_Comment = (5-isomer)
| CASNo1_Comment =5-异构体
| CASNo2_Ref = {{cascite|correct|CAS}}
| CASNo2_Ref = {{cascite|correct|CAS}}
| CASNo2 = 18861-78-4
| CASNo2 = 18861-78-4
| CASNo2_Comment = (6-isomer)
| CASNo2_Comment = 6-异构体
| UNII_Ref = {{fdacite|correct|FDA}}
| UNII_Ref = {{fdacite|correct|FDA}}
| UNII = 7BUU93N0HM
| UNII = 7BUU93N0HM
| UNII1_Ref = {{fdacite|correct|FDA}}
| UNII1_Ref = {{fdacite|correct|FDA}}
| UNII1 = I223NX31W9
| UNII1 = I223NX31W9
| UNII1_Comment = (5-isomer)
| UNII1_Comment =5-异构体
| UNII2_Ref = {{fdacite|correct|FDA}}
| UNII2_Ref = {{fdacite|correct|FDA}}
| UNII2 = 360X6ZS9ZB
| UNII2 = 360X6ZS9ZB
| UNII2_Comment = (6-isomer)
| UNII2_Comment =6-异构体
| PubChem = 131730176
| PubChem = 131730176
| PubChem_Comment = (mix)
| PubChem_Comment =混合物
| PubChem1 = 18730
| PubChem1 = 18730
| PubChem1_Comment = (5-isomer)
| PubChem1_Comment =5-异构体
| PubChem2 = 2762652
| PubChem2 = 2762652
| PubChem2_Comment = (6-isomer)
| PubChem2_Comment =6-异构体
| ChEBI_Ref = {{ebicite|correct|EBI}}
| ChEBI_Ref = {{ebicite|correct|EBI}}
| ChEBI = 37928
| ChEBI = 37928
第73行： 第73行：
| Section6 =
| Section6 =
}}
}}
'''{{PAGENAMEBASE}}'''（{{lang-en|Fluorescein isothiocyanate}}，缩写'''FITC'''），是一种[[荧光素]]衍生物类[[荧光]]染料，广泛应用于[[流式細胞術]]和[[熒光顯微鏡]]，激发峰在495nm，发射峰在519nm<ref>{{cite journal | pmc = 1455721| year = 1970| author1 = The TH| title = Conjugation of fluorescein isothiocyanate to antibodies: I. Experiments on the conditions of conjugation| journal = Immunology| volume = 18| issue = 6| pages = 865–873| last2 = Feltkamp| first2 = T. E.| pmid = 5310665}}</ref>。常用的FITC是两种[[异硫氰基]][[官能团]]位置异构体5-FITC和6-FITC的化合物<ref>{{cite journal | pmc = 1455722| year = 1970| author1 = The TH| title = Conjugation of fluorescein isothiocyanate to antibodies: II. A reproducible method| journal = Immunology| volume = 18| issue = 6| pages = 875–881| last2 = Feltkamp| first2 = T. E.| pmid = 4913804}}</ref>。
'''{{PAGENAMEBASE}}'''（{{lang-en|Fluorescein isothiocyanate}}，缩写'''FITC'''），是一种[[荧光素]]衍生物类[[荧光]]染料，广泛应用于[[流式細胞術]]和[[熒光顯微鏡]]，激发峰在495nm，发射峰在519nm<ref>{{cite journal | pmc = 1455721| year = 1970| author1 = The TH| title = Conjugation of fluorescein isothiocyanate to antibodies: I. Experiments on the conditions of conjugation| journal = Immunology| volume = 18| issue = 6| pages = 865–873| last2 = Feltkamp| first2 = T. E.| pmid = 5310665}}</ref>。常用的FITC是两种[[异硫氰酸酯|异硫氰酸基]][[官能团]]位置异构体5-FITC和6-FITC的化合物<ref>{{cite journal | pmc = 1455722| year = 1970| author1 = The TH| title = Conjugation of fluorescein isothiocyanate to antibodies: II. A reproducible method| journal = Immunology| volume = 18| issue = 6| pages = 875–881| last2 = Feltkamp| first2 = T. E.| pmid = 4913804}}</ref>。
<gallery>
<gallery>
File:Fluorescein-spectra2.svg|FITC的激发光谱和发射光谱
File:Fluorescein-spectra2.svg|FITC的激发光谱和发射光谱
</gallery>
</gallery>

==另见==
* [[TRITC]]（异硫氰酸四甲基罗丹明）


==参考文献==
==参考文献==
第82行： 第85行：


==外部链接==
==外部链接==
{{Fluorophore}}
{{DEFAULTSORT:Fluorescein Isothiocyanate}}
{{DEFAULTSORT:Fluorescein Isothiocyanate}}

[[Category:苯甲酸衍生物]]
[[Category:荧光酮染料]]
[[Category:异硫氰酸酯]]
[[Category:染色着色剂]]
[[Category:1950年代发现的物质]]

2024年3月20日 (三) 02:38的最新版本

异硫氰酸荧光素
别名 FITC
5-异构体：FITC-I
6-异构体：FITC-II
识别
CAS号 27072-45-3（混合物）  checkY
3326-32-7（5-异构体）  checkY
18861-78-4（6-异构体）  checkY
PubChem 131730176（混合物）
18730（5-异构体）
2762652（6-异构体）
ChemSpider 17686 5-异构体, 2043359 6-异构体
SMILES
 
  • c1cc2c(cc1N=C=S)C(=O)OC23c4ccc(cc4Oc5c3ccc(c5)O)O
InChI
 
  • 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChIKey MHMNJMPURVTYEJ-UHFFFAOYSA-N
ChEBI 37928
MeSH Fluorescein+isothiocyanate
性质
化学式 C21H11NO5S
摩尔质量 389.38 g·mol−1
密度 1.542 g/mL
若非注明，所有数据均出自标准状态（25 ℃，100 kPa）下。

异硫氰酸荧光素（英語：Fluorescein isothiocyanate，缩写FITC），是一种荧光素衍生物类荧光染料，广泛应用于流式細胞術和熒光顯微鏡，激发峰在495nm，发射峰在519nm[1]。常用的FITC是两种异硫氰酸基官能团位置异构体5-FITC和6-FITC的化合物[2]。

  • FITC的激发光谱和发射光谱
    FITC的激发光谱和发射光谱

另见

[编辑]
  • TRITC（异硫氰酸四甲基罗丹明）

参考文献

[编辑]
  1. ^ The TH; Feltkamp, T. E. Conjugation of fluorescein isothiocyanate to antibodies: I. Experiments on the conditions of conjugation. Immunology. 1970, 18 (6): 865–873. PMC 1455721 可免费查阅. PMID 5310665. 
  2. ^ The TH; Feltkamp, T. E. Conjugation of fluorescein isothiocyanate to antibodies: II. A reproducible method. Immunology. 1970, 18 (6): 875–881. PMC 1455722 可免费查阅. PMID 4913804. 

外部链接

[编辑]
检索自“https://zh.wikipedia.org/zhwiki/w/index.php?title=异硫氰酸荧光素&oldid=81963038”