User:Magggey/Nanobiomechanics

Nanobiomechanics

Nanobiomechanics (also bionanomechanics) is an emerging field in nanoscience and biomechanics that combines the powerful tools of nanomechanics to explore fundamental science of biomaterials and biomechanics.

Since the introduction by its founder Yuan-Cheng Fung, the field of biomechanics has become one of the branches of mechanics and bioscience. For many years, biomechanics has examined tissue. Through advancements in nanoscience, the scale of the forces that could be measured and also the scale of observation of biomaterials was reduced to "nano" and "pico" level. Consequently, it became possible to measure the mechanical properties of biological materials at nanoscale. This is relevant to improve tissue engineering processes and cellular therapy.

Most of the biological materials have different hierarchical levels, and the smallest ones refer to the nanoscale. For example, bone has up to seven levels of biological organization, and the smallest level, i.e., single collagen fibril and hydroxylapatite minerals have dimensions well below 100 nm. Therefore, being able to probe properties at this small scales provides a great opportunity for better understanding the fundamental properties of these materials. For example, measurements have shown that nanomechanical heterogeneity exists even within single collagen fibrils as small as 100 nm.

One of the other most relevant topics in this field is measurement of tiny forces on living cells to recognize changes caused by different diseases, including disease progression. For example, it has been shown that red blood cells infected by malaria are 10 times stiffer than normal cells. Likewise, it has been shown that cancer cells are 70 percent softer than normal cells. Early signs of aging cartilage and osteoarthritis has been shown by looking at the changes in the tissue at the nanoscale.

Methods, instrumentation, and application

The common methods in nanobiomechanics include atomic force microscopy (AFM), nanoindentation(link), and application of nanoparticles(link).[kilpatrick,tai,septiadi]

Atomic Force Microscopy

For a description of atomic force microscopy, see atomic force microscope.

AFM has been used to study the nanoscale level of the cytoskeleton and its components, the extracellular matrix, and the cell's environment. Understanding the cell's mechanics, including at a nanoscale level, is highly connected to understanding these molecules and structures. As all of this affects how the cell behaves, it is beneficial for tissue engineering.[kilpatrick] One example of this is when researchers applied tapping mode AFM to study repair bone from genetically modified mesenchymal cells. Via this method, they were able to image structures in the bone on a nano scale that suggested collagen was present.[tai]

AFM has also been applied to measure the mechanical properties of proteins and other biomolecules in a variety of conditions through extension and compression experiments.[ikai] Further, it has been applied to the mapping of cells' and membranes' mechanical properties, mechanotransduction, how cells adhere or detach based on the surface they are on and their own molecules, and the stiffness of cells. [kilpatrick]

As metastatic cells have been shown to be softer than benign cells using AFM, the mechanics of cancer cells may be useful to diagnose cancer. [kilpatrick]

Nanoindentation

For a description of nanoindentation, see nanoindentation(link).

Tai et al. applied nanoindentation to study repair bone from genetically modified mesenchymal cells. They compressed a probe with a nanometer radius into both native and repair bone and used it to study the deformability of the tissue. This gave them insight into mechanical properties of the bone, including its stiffness. Nanoindentation allowed them to study the bone’s compressibility through loading and unloading curves.[tai]

Further, nanoindentation may be combined with other methods in specific studies. One example includes AFM nanoindentation, which has been applied to study subcellular components in living cells.[chen]

Application of Nanoparticles

Nanoparticles both affect cells on a nanoscale level, and are one method of studying the mechanical properties of cells and biomaterials on the nanoscale level. Nanoparticles affect how cells adhere to substrates, and the cell’s stiffness. They also impact components of the cell’s cytoskeleton which in turn affect cell motility as they bind and interact with structures such as receptors and RNA.[septiadi]

As these nanoparticles affect the nanobiomechanics of cells, they are valuable tools to study them. For example, nanoparticles have been embedded on the surfaces of structures to alter the nanotopographical environment, and affected how the cell behaved. This included how cells spread, how cytoskeletal components assemble, and how cells attach. Some included nanoparticles have magnetic properties, and have been used in conjunction with magnetic fields for detailed control of cellular surfaces and other studies. [septiadi]

Nanoparticles are useful in studying the ways cells adapt physical forces into biochemical signals, and the mechanical properties of cellular constituents. They have also been used in processes such as particle tracking microrheology.[septiadi]

Materials

Examples of relevant materials are bone and its hierarchical constituents such as single collagen fibrils, single living cells, actin filaments and microtubules.

Lead

• Clean up the definition section. Maybe get rid of the stuff on Yuang? Or leave? I’m not sure yet. Maybe I just won’t focus on that, since it isn’t biophysics.
• The field is relevant to improve tissue engineering processes and cellular therapy. Nanobiomechanics also helps us better understand the progression of disease. (2, conclusion)

Article body (Points I want to add in)

• Methods and Instrumentation OVERALL HEADER, THEN a little chunk on each. Include: Multiple methods are used together to gain a deeper understanding of the biomechanics of cells or other biomaterials. [cite]
  • Nanoparticles
    • Nanoparticles both affect cells on a nanoscale level, and are one method of studying the mechanical properties of cells and biomaterials on the nanoscale level. Nanoparticles affect how cells adhere to substrates, and the cell’s stiffness. They also impact components of the cell’s cytoskeleton which in turn affect cell motility as they bind and interact with structures such as receptors and RNA. (4)
    • As these nanoparticles affect the nanobiomechanics of cells, they are valuable tools to study them. For example, nanoparticles have been embedded on the surfaces of structures to alter the nanotopographical environment, and affected how the cell behaved. This included how cells spread, how cytoskeletal components assemble, and how cells attach. Some included nanoparticles have magnetic properties, and have been used in conjunction with magnetic fields for detailed control of cellular surfaces and other studies. (4)
    • Nanoparticles are useful in studying the ways cells adapt physical forces into biochemical signals, and the mechanical properties of cellular constituents. (4)
    • Nanoparticles have also been used in processes such as particle tracking microrheology.  (4)
  • Nanoindentation
    • Tai et al. applied nanoindentation to study repair bone from genetically modified mesenchymal cells. They compressed a probe with a nanometer radius into both native and repair bone and used it to study the deformability of the tissue. This gave them insight into mechanical properties of the bone, including its stiffness. Nanoindentation allowed them to study the bone’s compressibility through loading and unloading curves. (6, Figure 6 description and “nanoindentation” heading.)
  • AFM
    • Tai et al. applied tapping mode AFM to study repair bone from genetically modified mesenchymal cells. Via this method, they were able to image structures in the bone on a nano scale. At this scale, they imaged structures that suggested collagen was present. (6, Figure 4 description). IS THIS nanobioMECHANICS though? I think so, just pull in how that’s so close to ECM or something from the other article: Understanding the cell’s mechanics is highly connected to understanding the cytoskeleton and its components, which AFM can help study. This is also useful for understanding the environment of the cell and the extracellular matrix, which affects how the cell behaves, and is beneficial for tissue engineering.(5)
    • AFM has also been applied to measure the mechanical properties of proteins and other biomolecules in a variety of conditions through extension and compression experiments. (3) ME: In presentation, include the stuff about the sandwich method, etc.
    • AFM has also been applied to the mapping of cells mechanical properties, mechanotransduction, how cells adhere or detach from things based on the surface they are on and their own molecules, and the stiffness of cells. (5)
    • Mechanics of cancer cells may be useful to diagnose cancer, as metastatic cells have been shown to be softer than benign cells using AFM. (5)
    • Some AFM approaches allow researchers to gain more information at the same time, such as multiharmonic imaging modes to image cells, that involves harmonics and may be applied to mapping “nanomechanical properties of cells and biological membranes”. (5)
  • Nanoindentation and AFM together
    • AFM nanoindentation is especially useful for studying subcellular components in living cells. Some have combined AFM and nanoindentation tools to get their results. (2)
    • These techniques are often applied together for nanobiomechanical studies, such as when Tai et al. applied it to study repair bone in ways not available through other biomechanical techniques. (6, I think in conclusion.)
  • Other applications
    • Article 1
    • One of the earliest articles discussing nanobiomechanics involved using it in a model to reconstruct physical values of breast tissue samples from ulltrasound data as a potential diagnostic tool for invasive ductal carcinoma compared to healthy breast tissue. In their sample, the nanobiomechanic model showed statistically significant differences between the tissues. This demonstrated that bionanomechanics may be helpful for further screening and diagnostic purposes for cancer.(1)

References

First Title: Mechanical spectral signatures of malignant disease? A small-sample, comparative study of continuum vs. nano-biomechanical data analyses

Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3851619/pdf/DM18-04-874157.pdf

From 2002 Primary Research.

One of the first articles, talks about the potential for using biomechanics to help diagnose breast cancer. Continuum mechanics vs. biomechanics in theoretical models. I think it is to analyze the ultrasound data using the models, and then seeing what is significant. Nanobiomechanics model was significant, but the sample size was very small, so was more saying, “this may be a good area for further research.”

Second Title: Nanobiomechanics of living cells: a review

Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982446/

From April 2014, Review article

Focuses on nanoindentation method

Third Title: Nanobiomechanics of proteins and biomembrane

Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2610188/

PMID: https://www.ncbi.nlm.nih.gov/pubmed/18339603 NUMBER: 18339603

From: 2008, Review article

Basically, their point is a review of work in their lab that has focused on applying nanobiomechanics to proteins, specifically in AFM.

Fourth Title:Nanoparticle–Cell Interaction: A Cell Mechanics Perspective

Link: https://onlinelibrary.wiley.com/doi/10.1002/adma.201704463

PMID: PMID: 29315860

DOI: https://doi.org/10.1002/adma.201704463

From: 2018 pretty sure

It looks like talks about a lot of different technologies in nanobiomechanics. This will be super helpful in expanding the sections I want to.

Fifth Title: Nanomechanics of Cells and Biomaterials Studied by Atomic Force Microscopy

Link: https://onlinelibrary.wiley.com/doi/10.1002/adhm.201500229

PMID: 26200464

DOI: https://doi.org/10.1002/adhm.201500229

From: 2015, Review

This will help me expand and maybe create a section with AFM using the other primary research article. Or maybe a tissue engineering section. Not 100% sure what direction I’ll take there yet.

Sixth Title: Nanobiomechanics of repair bone regenerated by genetically modified mesenchymal stem cells

Link: https://pubmed.ncbi.nlm.nih.gov/18620480/

PMID: 18620480  DOI: 10.1089/ten.tea.2007.0241

Could help me expand on the portion of the stub about bone. And it references nanonindentation. So that could be helpful.