Wikipedia:Peer review/Glutathione S-transferase/archive1

Glutathione S-transferase

This peer review discussion has been closed.
I've listed this article for peer review because I am one of seven students in this graduate-level course, and opening this peer review is part of my assignment. Please suggest how I could help this article meet the good article criteria. The assignment ends on May 8, so responses received by May 5 will allow me time to address your comments. Achieving GA status is not part of my grade, but my responses here and the edits I make to the article to address your suggestions will be evaluated by my professor.

Thanks, Jnims (talk) 21:00, 22 April 2013 (UTC)

From Biosthmors

• Thanks for your contributions! Sorry I didn't get comments up for you sooner. I hope these comments help. I'm going to be looking for ways to improve the article, so I'll share my thoughts that will hopefully guide you to making improvements to the article.
  • Thanks!
• The WP:First sentence is supposed to be a concise definition, if possible. Could we reword to define the subject, by perhaps including some characteristic function and/or mechanism of the protein family? Currently we have: "The glutathione S-transferase (GST) family of enzymes (previously known as ligandins) are composed of many cytosolic, mitochondrial, and microsomal—also known as MAPEG—proteins." Is it also necessary for us to introduce the concept of MAPEG proteins in a definition? My guess is that that could wait. Also, why include "microsomal"? I see from the Wikipedia article that those are only laboratory created "organelles". (see also my comment below that starts with "I now see")
  • I have edited the first sentence (and paragraph) substantially, following your recommendations.
• In the WP:Lead I see that the sentence "GSTs can constitute up to 10% of cytosolic protein in some mammalian organs" is cited to a 1989 source. Could we get something much more recent? Or at least cite a recent secondary source if this is an important primary source you would like to preserve?
  • I have added a secondary source.
• Could we clarify and link that glutathione is the reducing agent in the reaction? Right now we have "reduced glutathione", which reads a bit awkwardly to me. In my mind, it implies that glutathione has to be reduced first before it can be used in the reaction.
  • Done.
• Would the word "isoenzyme" be appropriate for the first sentence/definition? What about the word "isoform"?
  • Done.
• Does the literature use the terms Alpha, Mu, Pi, Theta, Zeta and Omega. My guess would be that the letters themselves predominate. If so, can we include the symbols or replace the words with the letters?
  • Most of the sources I've found use the Latin letter abbreviation (e.g., "p" for pi) rather than the Greek letter, so I chose to use the full name for the letter.
• I now see "Protein sequence and structure are important additional classification criteria for the three superfamilies (cytosolic, mitochondrial, and MAPEG) of GSTs". I'm not sure what's what here. Clarify?
  • I hope my previous edits have clarified this; I'm not sure what's unclear about this sentence, but would be happy to change it if you could provide more detail.
• Well that is all I have time and energy for at the moment! I hope these comments have been helpful and that these ideas might give you insight on how to improve other parts of the page. Feel free to contact me if you have any questions. Best wishes. Biosthmors (talk) 12:11, 6 May 2013 (UTC)
  • Thank you very much! Jnims (talk) 01:10, 8 May 2013 (UTC)

Ian's comments

-Would it be good to add the organs? "GSTs can constitute up to 10% of cytosolic protein in some mammalian organs"