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谷胱甘肽还原酶

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谷胱甘肽还原酶
PDB rendering based on 1GRE.
有效结构
PDB 直系同源检索：PDBe, RCSB
标识
代号 GSR; MGC78522
扩展标识 遗传学：138300 鼠基因：95804 同源基因：531 GeneCards: GSR Gene
EC編號 1.8.1.7
RNA表达模式
更多表达数据
直系同源体
物种 人类 小鼠
Entrez 2936 14782
Ensembl ENSG00000104687 ENSMUSG00000031584
UniProt P00390 n/a
mRNA序列 NM_000637.3 NM_010344.4
蛋白序列 NP_000628.2 NP_034474.4
基因位置 Chr 8:
30.54 – 30.59 Mb		 Chr 8:
34.76 – 34.81 Mb
PubMed查询 [2] [3]

谷胱甘肽还原酶[1]（英語：Glutathione reductase，EC 1.8.1.7）是一种将氧化型谷胱甘肽（GSSG）还原成为硫醇型谷胱甘肽的酶，而谷胱甘肽是一种重要的细胞抗氧化剂[2][3]。

对于一摩尔的氧化型谷胱甘肽（GSSG），需要一摩尔的NADPH将氧化型谷胱甘肽还原为还原型谷胱甘肽。这种酶形成一个FAD结合的同型二聚体。谷胱甘肽还原酶在所有界中都是进化保守的。在细菌、酵母与动物中，编码谷胱甘肽还原酶的基因是同一种；然而，在植物基因组中，有两个被编码的谷胱甘肽还原酶。果蝇与锥体虫甚至都没有编码谷胱甘肽还原酶的基因[4]。在这些生物中，谷胱甘肽还原酶的还原是分别由硫氧还蛋白以及锥虫胱甘肽系统来完成的[4][5]。

以NADPH为氢供体、氧化型谷胱甘肽为氢受体的还原酶，反应生成NADP+和还原型谷胱甘肽。

参考文献

[编辑]
  1. ^ [1][永久失效連結]
  2. ^ Meister A. Glutathione metabolism and its selective modification. J. Biol. Chem. 1988, 263 (33): 17205–8 [2011-09-19]. PMID 3053703. （原始内容存档于2009-08-17）. 
  3. ^ Mannervik B. The enzymes of glutathione metabolism: an overview. Biochem. Soc. Trans. 1987, 15 (4): 717–8. PMID 3315772. 
  4. ^ 4.0 4.1 Kanzok SM, Fechner A, Bauer H, Ulschmid JK, Müller HM, Botella-Munoz J, Schneuwly S, Schirmer R, Becker K. Substitution of the thioredoxin system for glutathione reductase in Drosophila melanogaster. Science. 2001, 291 (5504): 643–6. PMID 11158675. doi:10.1126/science.291.5504.643. 
  5. ^ Krauth-Siegel RL, Comini MA. Redox control in trypanosomatids, parasitic protozoa with trypanothione-based thiol metabolism. Biochim Biophys Acta. 2008, 1780 (11): 1236–48. PMID 18395526. doi:10.1016/j.bbagen.2008.03.006. 

深入阅读

[编辑]
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  • Krohne-Ehrich G, Schirmer RH, Untucht-Grau R. Glutathione reductase from human erythrocytes. Isolation of the enzyme and sequence analysis of the redox-active peptide.. Eur. J. Biochem. 1978, 80 (1): 65–71. PMID 923580. doi:10.1111/j.1432-1033.1977.tb11856.x. 
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  • Krauth-Siegel RL, Blatterspiel R, Saleh M; et al. Glutathione reductase from human erythrocytes. The sequences of the NADPH domain and of the interface domain.. Eur. J. Biochem. 1982, 121 (2): 259–67. PMID 7060551. doi:10.1111/j.1432-1033.1982.tb05780.x. 
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  • Stoll VS, Simpson SJ, Krauth-Siegel RL; et al. Glutathione reductase turned into trypanothione reductase: structural analysis of an engineered change in substrate specificity. Biochemistry. 1997, 36 (21): 6437–47. PMID 9174360. doi:10.1021/bi963074p. 
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  • Berry Y, Truscott RJ. The presence of a human UV filter within the lens represents an oxidative stress. Exp. Eye Res. 2001, 72 (4): 411–21. PMID 11273669. doi:10.1006/exer.2000.0970. 
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